Oxidative Stress Test Kit
The oxidative stress test kit is designed to examine oxidative stress levels in animals, humans, and samples in contact with drugs and food. Oxidative stress occurs when your antioxidant levels are low, and there is an imbalance between free radicals. This imbalance can result in the development of various illnesses like diabetes.
Although oxidative stress has a few benefits, according to a MDPI 2021 medical research, oxidative stress plays a vital role in the progression of diseases and pathogenesis.
However, with the advancement of biotechnology, healthcare has progressed significantly with oxidative stress test kits. They play a significant role in the early detection of oxidative stress.
In this article, I’ll elaborate on the benefits of an oxidative stress test kit and its key components along with the required precautions.
What is an Oxidative Stress Kit and How Does It Work?
An oxidative stress test kit is a diagnostic tool to measure levels of oxidative stress in a body. In the last 30 years, several commercial assay kits have been made to measure oxidative stress. These kits ensure an easy method for immuno-detection of carbonyl-method proteins that ensures reproducible quantities.
Most oxidative stress kits measure stable molecular products formed from RONS reactions with biomolecules. These include:
- Lipid peroxidation end products: Isoprostanes, malondialdehyde (MDA)
- Oxidized proteins: protein carbonyls, nitrotyrosine
- Nucleic Acid: 8-hydroxy-2’-deoxyguanosine (8-OHdG)
Oxidative stress kits collect a biological sample (blood, urine, or tissue) and prepare it according to the kit’s instructions. Its functioning depends on the type of biomarker it measures, such as:
| DNA Oxidative Damage | This is a urine test for 8-hydroxy-2'-deoxyguanosine as an indicator of oxidative stress and damage. |
| Cellular Nutrition Assay | A blood sample is processed to determine the micronutrient and Redox/Antioxidant status and to therefore identify the patient's needs in terms of nutrition. |
| Specific Antioxidant Test | A patient's blood sample is analyzed to identify glutathione, superoxide dismutase, or catalase which points out the antioxidant presence in a human's body. |
| Lipid Peroxidation Test | Measure malondialdehyde (MDA) and other by-products of lipid peroxidation using assays like TBARS (Thiobarbituric Acid Reactive Substances). |
| Antioxidant Capacity Test | Performed on various sample types including blood, urine, or saliva. It measures the overall antioxidant capacity of a sample, reflecting the sum of all antioxidants present. |
| Sweat Analysis | Measures biomarkers such as isoprostanes and malondialdehyde by utilizing advanced biochemical assays and electrochemical sensors in wearable devices. |
Benefits of Oxidative Stress Test Kits
The following are the benefits of oxidative stress test kits:
- According to MDPI Journal research, tracking markers of oxidative and inflammatory activity may allow earlier and more effective strategies to prevent the development of non-communicable diseases (NCDs).
- By assessing precise free-radical testing, healthcare providers can organize treatment plans for individual patients at an early stage.
- The kits are designed to provide consistent and reproducible results, ensuring that the measurements are accurate and reliable.
- Oxidative test kits identify an antioxidant deficiency, allowing specific diet and treatment plans to improve health.
- According to Science Direct research, the biomarkers of oxidative stress test kits are useful in measuring the progression of various diseases.
- The oxidative stress kits offer a view of oxidative stress inside and outside cells in various cellular compartments.
- These kits can be used in multiple research fields such as cancer, neurodegenerative diseases like Parkinson’s and Alzheimer’s, etc.
Types of Oxidative Stress Test Kits
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| Type of Kit | Use |
| OxyIHC Oxidative Stress Detection Kit | This kit uses chemical and immunological reagents important to detect protein oxidation in certain tissues including a variety of organs as well as animal species. |
| 8-ohdg Ultrasensitive Elisa Assay Kit | Oxidative stress ELISA assay kits are sensitive and particular assay tests for quantifying the concentration of C-reactive Protein; Creatinine. |
| Catalase Activity Assay | Catalase enzyme goes through a reaction giving rise to a complex compound with chromogen. The reaction produces a purple color that increases with the catalase activity. It can be determined by simple spectrophotometer measurements.  |
| BodyK’s Digital Screening | BodyK measures the conductance average of a sweat peak, actually calculating the electrical properties of sweat. Sweat is full of biomarkers, including those that may indicate oxidative stress. It is via the measurement of these properties that this device can estimate the level of oxidative stress within the body. |
| Glutathione Assays | Glutathione aids in protecting cells from damage caused by ROS. Patients associated with oxidative stress have low levels of glutathione. |
| OxyBlot Protein Detection Kit | The OxyBlot protein detection kit supplies the necessary reagents for the immunoblot detection of carbonyl groups. The groups have been introduced through oxidative reactions with ozone, nitrogen oxides, or metal-catalyzed oxidation. |
Role Of Oxidative Stress Test Kits In Diagnosis
Oxidative stress test kits are crucial and may help to diagnose the following diseases:
- Cardiovascular Diseases: Oxidative stress is one of the major factors contributing to the development of atherosclerotic plaques and becomes an important risk factor in conditions like coronary artery disease and hypertension.
- Diabetes: Chronically elevated blood sugar levels cause increased oxidative stress, which is linked to underlying complications such as retinopathy, damage to the kidneys, and diabetic neuropathy. Studies have revealed that both chronic and acute hyperglycemia have been shown to induce oxidative stress, causing potential nerve damage and progression of diabetic neuropathy.
- Chronic Inflammatory Diseases: A role for oxidative stress in a variety of diseases, such as rheumatoid arthritis and inflammatory bowel disease, is linked to the aggravation of inflammation and tissue damage.
Difference Between In Vitro and In Vivo Testing
It is essential to understand the difference between In vivo and In vitro testing using oxidative stress test kits:
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| In Vitro Testing | In Vivo Testing |
| In vitro tests refer to experiments conducted outside a living organism in a controlled laboratory environment. | In vivo tests are experiments conducted within living organisms. |
| In vitro tests are performed on cell cultures, tissues, or biological molecules from the organisms. | In vivo tests take place in the natural environment. |
| In vitro tests allow a high level of control over experimental conditions. | In vivo tests give a better understanding of how oxidative stress affects the organism. |
| Oxidative stress kits used in vitro would measure the production of ROS and antioxidant levels. | Oxidative tests in vivo might involve measuring ROS levels in blood or tissues. |
Precautions
- Handle samples properly and check for kit calibration and expiration.
- The results can always vary, so it is always better to consult a professional.
- Potential side effects might include minor discomfort or allergic reactions.
- Do not use it in case of pregnancy, severe illness, or interference with certain medication.
People Also Ask
How Do You Test for Oxidative Stress?
One can test for oxidative stress by using a diagnostic kit to measure biomarkers like lipid peroxidation products, oxidized proteins, and nucleic acids in biological samples.
How Do You Measure Oxidative Stress Levels?
Oxidative stress levels are measured by analyzing stable molecular products formed from reactions with reactive oxygen and nitrogen species (RONS) in biological samples.
What Is the Best Marker for Oxidative Stress?
The best marker for oxidative stress is 8-hydroxy-2’-deoxyguanosine (8-OHdG), which indicates oxidative damage to DNA.
How Do You Know If You Have Oxidative Stress?
You may have oxidative stress if a test kit identifies low antioxidant levels and high biomarkers like malondialdehyde (MDA) or protein carbonyls in your samples.
What Is an Oxidative Stress Test Kit?
An oxidative stress test kit is a tool that measures oxidative stress markers in biological samples, helping detect oxidative stress levels and guide treatment plans.
Oxidative stress test kits are important for detecting oxidative stress and its related conditions and they offer valuable insights into disease progression. However, it is crucial to use it accurately, handle it properly, and consult with a professional to ensure reliable results.
References
https://www.webmd.com/a-to-z-guides/what-is-oxidative-stress
https://www.mdpi.com/2076-3921/10/2/201
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2642810
https://www.mdpi.com/2076-3921/10/1/15
